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Literature summary extracted from

  • Otero, L.H.; Beassoni, P.R.; Domenech, C.E.; Lisa, A.T.; Albert, A.
    Crystallization and preliminary X-ray diffraction analysis of Pseudomonas aeruginosa phosphorylcholine phosphatase (2010), Acta Crystallogr. Sect. F, 66, 957-960.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.75 gene pchP327, recombinant expression with a N-terminal fusion to an MGSSHHHHHHSSGLVPRGSH tag in Escherichia coli BL21, expression of N-terminal His6-tagged PchP in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.75 purified recombinant enzyme, sitting drop vapour diffusion method, 250 nl of 10 mg/ml protein in 200 mM NaCl, 10 mM Tris-HCl, pH 8.0, are mixed with 250 nl well solution and are equilibrated against 0.5 ml well solution containing 0.1 M Bis-Tris, pH 5.5, 0.2 M MgCl2, 25% PEG 3350, producing clustered plate-like microcrystals, or by microbatch method mixing 0.001 ml of protein solution containing 10 mg/ml in 200 mM NaCl, 20 mM Bis-Tris, pH 5.5, with 0.002 ml precipitant solution, cryoprotection by a quick soak in 0.1 M Bis-Tris pH 5.5, 0.2 M MgCl2, 25% PEG 3350, 15% glycerol, X-ray diffraction structure determination and analysis at 2.7 A resolution Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.75 Zn2+ inhibition produced by Zn2+ at pH 7.4 represents a change from octahedral to tetrahedral coordination geometry which is produced by hydrolysis of the Zn-hexacoordinated complex Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.75 Cu2+ dependent on divalent cations Mg2+, Zn2+ or Cu2+ Pseudomonas aeruginosa
3.1.3.75 Mg2+ dependent on divalent cations Mg2+, Zn2+ or Cu2+ Pseudomonas aeruginosa
3.1.3.75 Zn2+ dependent on divalent cations Mg2+, Zn2+ or Cu2+ Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.75 37000
-
 2 * 37000, about, recombinant His6-tagged enzyme, SDS-PAGE Pseudomonas aeruginosa
3.1.3.75 60000
-
 about, recombinant His6-tagged enzyme, gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.75 phosphocholine + H2O Pseudomonas aeruginosa
-
 choline + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.75 Pseudomonas aeruginosa Q9HTR2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.75 recombinant N-terminally His6-tagged PchP from Escherichia coli by nickel affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.75 phosphocholine + H2O
-
 Pseudomonas aeruginosa choline + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.75 dimer 2 * 37000, about, recombinant His6-tagged enzyme, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.3.75 More PchP belongs to the HAD superfamily Pseudomonas aeruginosa
3.1.3.75 PChP
-
 Pseudomonas aeruginosa
3.1.3.75 phosphorylcholine phosphatase
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
3.1.3.75 physiological function possible importance of PchP in the pathogenesis of Pseudomonas aeruginosa Pseudomonas aeruginosa